• 4X CAPITAL™ 1-Step qRT-PCR Green Master Mix (HRox)

4X CAPITAL™ 1-Step qRT-PCR Green Master Mix (HRox)

  • Biotechrabbit
  • BR0502500
  • Na skladě
  • 4 186,00Kč
  • 4 106,00Kč

Dostupné možnosti

- OR -
Tento výrobek má minimální množství 1

Dopravné a balné

Transportní podmínky                                  cena se připočítává k položkám v objednávce

mražené na suchém ledu (dry ice)                   890 Kč bez DPH na zásilky v celkové ceně do 45 000 Kč bez DPH, nad tuto hodnotu je dopravné a balné zdarma


Features

  • Convenient mix for quantification of RNA templates
  • Sensitive and specific amplification with rapid extension rate for early Ct values
  • Excellent linearity across a wide range of RNA dilution

Applications

  • One step qRT-PCR from mRNA, total RNA and viral RNA targets
  • For use with standard and fast qPCR platforms
  • BR0502501200 reactions of 20 µl1 ml CAPITAL qPCR Green Master Mix (1step) HROX
    200 µl RTase with RNase Inhibitor

    BR05025021000 reactions of 20 µl5 × 1 ml CAPITAL qPCR Green Master Mix (1step) HROX
    5 × 200 µl RTase with RNase Inhibitor

    BR05025034000 reactions of 20 µl4 kits BR0502502 CAPITAL 1-Step qRT-PCR Green Master Mix HROX (1000 rxn each)
  • biotechrabbit CAPITAL™ qRT-PCR Green Mix allows sensitive and specific cDNA synthesis and qPCR in a single tube for quantifying mRNA, total RNA and viral RNA sequences. Extremely low-copy-number targets can be detected with high efficiency over several logs of template concentration.

    CAPITAL qRT-PCR Green Mix uses proprietary reverse transcriptase technology and buffer chemistry for efficient cDNA synthesis and QPCR in a single tube. To enable the use of the kit on qPCR platforms with different reference dye concentration requirements, three kit formats are available: a one-step kit containing no ROX, as well as LRox and HRox versions containing ROX in the corresponding concentratios.

     

    Info: Recommended annealing temperature is 2°C above primer Tm (use gradient PCR to optimize the annealing temperature).